Surgical procedure for acellular vascular xenografts testing in sheep carotid artery
Abstract
Objective: The objective of this study is to demonstrate the safety and reproducibility of our surgical technique for implanting decellularized vascular xenografts in the carotid artery of sheep.
Methods: Acellular porcine carotid arteries were implanted as interposition xenografts in seven sheep. An intravascular shunt was used for cerebral protection, and a flowmeter was utilized to assess graft performance.
Results: There were no intraoperative deaths or postoperative neurological complications observed. Acute graft thrombosis occurred in one sheep during surgery, but was successfully managed with thrombectomy to restore blood flow. Post-implantation flowmetry and Doppler ultrasound confirmed graft functionality.
Conclusions: Our study demonstrates the successful application of our surgical method for implanting decellularized vascular xenografts in the carotid artery of sheep. The implanted grafts maintained patency, normal blood flow, and favorable wound healing and neurological outcomes post-surgery.
Keywords: vascular xenograft, carotid artery, ovine model
Copyright (c) 2024 Marius Mihai Harpa, Anitei David Emanuel, Simionescu Dan Teodor, Truta Raluca Ionela, Al-Hussein Hamida, Cotfas Ionela, Ghiragosian Claudiu, Banceu Marian Cosmin, Capilna Tudor, Suciu Horatiu, Al-Hussein Hussam
This work is licensed under a Creative Commons Attribution 4.0 International License.
