Surgical procedure for acellular vascular xenografts testing in sheep carotid artery

  • Marius Mihai Harpa Regenerative Medicine Laboratory, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 540142, Romania
  • Puscas Alexandra Iulia George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 540142, Romania
  • Anitei David Emanuel Emergency Institute for Cardiovascular Diseases and Transplantation Targu Mures, 540136, Romania
  • Simionescu Dan Teodor Clemson University, South Carolina
  • Truta Raluca Ionela Regenerative Medicine Laboratory, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 540142, Romania
  • Al-Hussein Hamida Regenerative Medicine Laboratory, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 540142, Romania
  • Cotfas Ionela Regenerative Medicine Laboratory, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 540142, Romania
  • Ghiragosian Claudiu George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 540142, Romania
  • Banceu Marian Cosmin George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 540142, Romania
  • Capilna Tudor George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 540142, Romania
  • Suciu Horatiu George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 540142, Romania
  • Al-Hussein Hussam George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 540142, Romania
Keywords: vascular xenograft, carotid artery, ovine model

Abstract

Objective: The objective of this study is to demonstrate the safety and reproducibility of our surgical technique for implanting decellularized vascular xenografts in the carotid artery of sheep.

Methods: Acellular porcine carotid arteries were implanted as interposition xenografts in seven sheep. An intravascular shunt was used for cerebral protection, and a flowmeter was utilized to assess graft performance.

Results: There were no intraoperative deaths or postoperative neurological complications observed. Acute graft thrombosis occurred in one sheep during surgery, but was successfully managed with thrombectomy to restore blood flow. Post-implantation flowmetry and Doppler ultrasound confirmed graft functionality.

Conclusions: Our study demonstrates the successful application of our surgical method for implanting decellularized vascular xenografts in the carotid artery of sheep. The implanted grafts maintained patency, normal blood flow, and favorable wound healing and neurological outcomes post-surgery.

Keywords: vascular xenograft, carotid artery, ovine model

Published
2024-07-23
How to Cite
1.
Harpa M, Iulia P, Emanuel A, Teodor S, Ionela T, Hamida A-H, Ionela C, Claudiu G, Cosmin B, Tudor C, Horatiu S, Hussam A-H. Surgical procedure for acellular vascular xenografts testing in sheep carotid artery. amm [Internet]. 23Jul.2024 [cited 8Oct.2024];70(3). Available from: https://ojs.actamedicamarisiensis.ro/index.php/amm/article/view/545
Section
Original article