How we did it- an easy and feasible experimental rat model of protective role of Lipid Emulsion in induced Local Anesthetic Systemic Toxicity

  • Alexandra Lazar University of Medicine, Pharmacy, Sciences and Technology "George Emil Palade" Tîrgu Mureș
  • Marcel Perian University of Medicine, Pharmacy, Sciences and Technology "George Emil Palade" Tîrgu Mureș
  • Bogdan Cordoș University of Medicine, Pharmacy, Sciences and Technology "George Emil Palade" Tîrgu Mureș
  • Mircea Gherghinescu University of Medicine, Pharmacy, Sciences and Technology "George Emil Palade" Tîrgu Mureș
  • Bianca Liana Grigorescu University of Medicine, Pharmacy, Sciences and Technology "George Emil Palade" Tîrgu Mureș
Keywords: Animal experiment, Local Anesthetic, Lipid Emulsion, Vascular Access, Toxicity

Abstract

Local Anesthetic Systemic Toxicity (LAST) is the most feared local anesthesia accident. As the cardiac arrest determined by LAST is mostly refractory to known resuscitation protocols, due local anesthetic blockade produced in the cardiac cells, the Lipid Emulsion (L.E) has been proved to be beneficial in resuscitating the cardiac arrest determined by local anesthetic

The aim for this presentation is to ease future studies on this topic, to ensure a starting point for next related research on LAST and LE mechanism of action.

Method

Under genaral anesthesia we induced Local Anesthetic Systemic Toxicity  to a rat model, by injecting Ropivacaine into the inferior vena cava. We monitored the cardiac activity of the subjects during the experiment. We used 4 groups of rats, control group- no intervention, lipid group- lipid emulsion was adminsitered, local anesthetic group- local anesthetic was administered and local anesthetic and lipid emulsion group- a dose of lipid emulsion was adminsitered before administering the local anesthetic.

Results

After a few attemps to incannulate teh peripheral veins we tried the more complex approach of inferior vena cava, which ensured a secure access which allowed us to repetedly adminster the local anesthetic and the lipid emulsion.

Conclusion

The presented experimental animal model of induced LAST and the protective effects of LE is one of the few described in the literature, is a reproducible model, feasible, simple, low cost and can be used as starting point in future LAST research.

Aknowledgement

This work was supported by the University of Medicine, Pharmacy, Science and Technology of Târgu Mureș Research Grant number 615/9/17.01.2019

Author Biographies

Alexandra Lazar, University of Medicine, Pharmacy, Sciences and Technology "George Emil Palade" Tîrgu Mureș

Department of Anesthesiology and Intensive Care- Lecturer

Marcel Perian, University of Medicine, Pharmacy, Sciences and Technology "George Emil Palade" Tîrgu Mureș

Physiology Departament- Lecturer

Bogdan Cordoș, University of Medicine, Pharmacy, Sciences and Technology "George Emil Palade" Tîrgu Mureș

Animal Experimental Station - Veterinarian

Mircea Gherghinescu, University of Medicine, Pharmacy, Sciences and Technology "George Emil Palade" Tîrgu Mureș

Department of Surgery- Assistant lecturer

Bianca Liana Grigorescu, University of Medicine, Pharmacy, Sciences and Technology "George Emil Palade" Tîrgu Mureș

Department of Pathophysiology- Lecturer

Published
2021-04-18
How to Cite
1.
Lazar A, Perian M, Cordoș B, Gherghinescu M, Grigorescu B. How we did it- an easy and feasible experimental rat model of protective role of Lipid Emulsion in induced Local Anesthetic Systemic Toxicity. amm [Internet]. 18Apr.2021 [cited 17Sep.2021];67(2). Available from: https://ojs.actamedicamarisiensis.ro/index.php/amm/article/view/40
Section
Original article