The effects of pearl oyster shell-derived bone grafts on TNF-α levels: an in vivo study in Cavia porcellus

  • Sri Oktawati
  • Nurlindah Hamrun Department of Oral Biology, Faculty of Dentistry, Hasanuddin University
  • Muthmainnah Muthmainnah
  • Dian Setiawati
  • Andi Sitti Hajrah Yusuf
Keywords: Bone graft, Pinctada maxima, pearl oyster shells, platelet-rich fibrin, TNF-α

Abstract

Objective: This study aims to assess the effect of bone graft materials derived from pearl oyster shells on the tumor necrosis factor-alpha (TNF-α) level. Methods: This research utilized a bone graft containing hydroxyapatite Pinctada maxima (HPM) powder obtained from pearl oyster shells. Material testing was conducted on 30 male guinea pigs aged 8-10 weeks, divided into three groups: the negative control group, the group treated solely with HPM, and the group treated with HPM combined with platelet-rich fibrin (PRF). Each treatment group was observed for 7 and 14 days. The results were analyzed using One-way ANOVA with a significance level of p<0.05. Results: The findings demonstrated a decrease in the average TNF-α levels across all groups. On observation days 7 and 14, the HPM group exhibited a significant difference compared to the negative control group, indicating reduced TNF-α levels. However, no significant difference was observed when comparing the HPM group with the HPM and PRF groups. Conclusions: Bone grafts derived from pearl oyster shells possess high levels of hydroxyapatite, and the addition of PRF into the bone graft effectively reduces TNF-α.

Published
2024-06-24
How to Cite
1.
Oktawati S, Hamrun N, Muthmainnah M, Setiawati D, Yusuf ASH. The effects of pearl oyster shell-derived bone grafts on TNF-α levels: an in vivo study in Cavia porcellus. amm [Internet]. 24Jun.2024 [cited 21Jul.2024];70(2). Available from: https://ojs.actamedicamarisiensis.ro/index.php/amm/article/view/398
Section
Original article